Listen In - Bitesize Bio Webinar Audios

Liquid chromatography-mass spectrometry (LC-MS) is a crucial technique in many life sciences, including proteomics, metabolomics, pharmaceutical analysis, clinical diagnosis, and forensic science. In this episode of Listen In, elevate your analysis and liquid chromatography. Learn what consumables, materials, and reagents can harm your LC-MS data for more accurate quantification using perfluoroalkyl substances (PFAS) as an example. See how contamination from consumables, equipment, reagents, and non-specific binding of samples to syringe filters can make LC-MS quantification inaccurate. Plus, explore the environmental impact of PFAS and how to choose syringe filters and other consumables for accurate quantification results. Watch the full presentation here: https://events.bitesizebio.com/contamination-and-recovery-of-pfas/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Holotomography has emerged as a helpful tool for imaging live specimens without additional pre-treatment, such as fixation, fluorescence labeling, and excitation. It can achieve long-term three-dimensional observations of live specimens for weeks without cellular damage caused by photoactivation. The high resolution (under 150 nm lateral) achieved through synthetic numerical aperture provides sufficient spatial information to distinguish various subcellular compartments such as nuclei, nucleoli, mitochondria, and lipid droplets. Furthermore, analysis of the measured individual cell data can elucidate the temporal 3D volumetric dynamics with the dry mass information. This episode presents the latest development of a low-coherence holotomography imaging system, HT-X1, and its numerous applications to different types of biological specimens, ranging from unicellular organisms to multicellular specimens. Plus, learn how to combine holotomography with downstream molecular analysis, such as cell biology, immunology, microbiology, material science, and in vitro diagnosis. Watch the full presentation here: https://events.bitesizebio.com/label-free-3d-live-cell-imaging-and/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Buffers are critical to nearly all our experiments. Poorly prepared buffers lead to failed Western blots, poor protein yields, flat binding curves—and wasted time. Understanding why you need buffers, knowing how to choose the right one, and troubleshooting buffer issues are fundamental skills you can apply to make all your experiments work better—and you only need to learn how to prepare them correctly once. In this episode, dive deep into the world of chemical buffers. Explore how to select the best buffer for your experiments, share practical tips on preparing buffer solutions, and troubleshoot any hiccups that might come your way. Plus, we'll demystify key concepts like pH and pKa. Watch the full presentation here: https://events.bitesizebio.com/back-to-buffer-basics-everything/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

You have written a research paper. What now? Your research can only have an impact if someone reads it. So, you must submit your article to a journal for publication. In this episode of Listen In, learn the nuts and bolts of scientific publication with a step-by-step tour of the whole process. Explore how to select the right journal and what tools are available to help make that decision. Plus, get advice on how to make the journal submission process go smoothly and how to take critical reviews and turn them into positives in your re-submission. Watch the full presentation here: https://events.bitesizebio.com/how-to-write-a-research-paper-iv-1/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/ This is part four of our How To Write a Research Paper series: I: Telling Your Story II: The Anatomy III: Common Problems And How To Overcome Them IV: Understanding The Publication Process

The best way to solve a problem is to avoid it in the first place. While journals can and do reject articles for scientific reasons, they also reject articles for structural issues. By taking a few simple steps, you can learn how to circumvent these difficulties for a smooth publication process. In this episode of Listen In, we'll go through some of the structural reasons that scientific journals reject articles, how to solve common structural issues, and additional steps you can take to help your journal reviewers. Watch the full presentation here: https://events.bitesizebio.com/how-to-write-a-research-paper-iii-1/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/ This is part three of our How To Write a Research Paper series: I: Telling Your Story II: The Anatomy III: Common Problems And How To Overcome Them IV: Understanding The Publication Process

All research papers have certain elements in common: Introduction, Methods, Results, and Conclusion. How can you structure your introduction to best frame the research question for your readers? Must the methods section read like a laundry list of reagents? Can you be dramatic in the results without losing objectivity? Is there any room in the concluding section to pose new conflicts? In this episode of Listen In, we will delve deeper into each of these sections to show you how to tailor them to your audience. Watch the full presentation here: https://events.bitesizebio.com/how-to-write-a-research-paper-ii-1/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/ This is part two of our How To Write a Research Paper series: I: Telling Your Story II: The Anatomy III: Common Problems And How To Overcome Them IV: Understanding The Publication Process

Rarely do we consider writing a scientific paper as a creative exercise. But what if that could change? The best way to communicate anything is through stories. The scientific process has all the elements inherent in storytelling: a rich background, a problem or conflict to solve, and a resolution. In this episode of Listen In, we will reframe the writing process to help you clearly and concisely communicate your research with great impact. Watch the full presentation here: https://events.bitesizebio.com/how-to-write-a-research-paper-i-1/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/ This is part one of our How To Write a Research Paper series: I: Telling Your Story II: The Anatomy III: Common Problems And How To Overcome Them IV: Understanding The Publication Process

Transgenic mouse models are important tools for testing therapeutic strategies. In this episode of Listen In, discover the benefits and essential considerations when characterizing and using transgenic mouse models to test therapeutic approaches to treat neurodegenerative diseases. Plus, explore a knock-in mouse model of frontotemporal dementia that harbors a common mutation in the progranulin gene to test an antisense oligonucleotide (ASO)-based therapeutic strategy. Watch the full presentation here: https://events.bitesizebio.com/using-antisense-to-characterize/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Explore how the latest electron microscopy techniques and volume imaging can capture fast, dynamic membrane and vesicle remodeling events during synaptic transmission at the ultrastructural level. Neurons communicate at specialized junctions, or synapses, via chemical messengers called neurotransmitters. Within a millisecond of neuronal stimulation, synaptic vesicles packed with neurotransmitters fuse with the presynaptic plasma membrane and release their contents. These neurotransmitters then bind and activate receptors on the postsynaptic membrane, resulting in cell-to-cell signal transmission. Then, to sustain this neurotransmission, new vesicles are recruited to the release sites. In this episode of Listen In, we dive into the powerful new technology to spatially and temporally investigate these dynamics. Watch the full presentation here: https://events.bitesizebio.com/visualizing-membrane-dynamics-by/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Experience the transformative power of unbiased, data-driven cell analysis with access to over 25 image-derived label-free parameters, empowering you to easily assess sample quality, optimize gating strategies, and validate rare events. In this episode, learn how automated image analysis combined with flow cytometry can help improve data accuracy and precision, optimize and verify gating strategies, and discover novel biology, such as cell–cell interactions. Join a panel of experts as they discuss the features, benefits, and applications of a paradigm-shifting technology in flow cytometry with the Invitrogen™ Attune™ CytPix™ Flow Cytometer. Watch the full presentation here: https://events.bitesizebio.com/benefits-of-imaging-data-in-flow/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Organoids are three-dimensional in vitro cultures derived directly from patient tissues or induced pluripotent stem cells (iPSC) and embryonic stem cells. They self-organize to generate structures that resemble the tissue of origin and recapitulate the key physiological functions of the parental organ. In this episode of Listen In, explore the benefits of organoids as infectious disease models and drug screening vehicles, learn how to start organoid culture in your laboratory, and discover a portfolio of organoids for your research applications. Watch the full presentation here: https://events.bitesizebio.com/jump-into-3d-organoids-basic/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

As a scientific researcher, you must present your work in an unbiased, original, and representative way. Without due care and attention, it is easy to drift over the boundary of what is acceptable and slip into habits that are unacceptable within the scientific community. This episode of Listen In with Cristy Gelling explores image manipulation. Many common types of image manipulation are classified by journals as scientific misconduct, even if you had no intent to deceive anyone. Learn what practices are considered fraud or misconduct, how image formats and manipulations affect your data, and ways to process images that ensure your science is sound and your results are publishable. Watch the full presentation here: https://events.bitesizebio.com/ethical-research-practice-3-image/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

As a scientific researcher, you must present your work in an unbiased, original, and representative way. Without due care and attention, it is easy to drift over the boundary of what is acceptable and slip into habits that are unacceptable within the scientific community. This episode of Listen In with Karen O'Hanlon Cohrt explores publishing negative results and what is acceptable. Get answers to key questions, such as what is a negative result, and when should we report such results? What is the difference between a negative result and an unexpected result? What is an outlier? Plus, learn when it is okay to exclude an outlier and when you should include outliers to report the variation in your experiment. Watch the full presentation here: https://events.bitesizebio.com/ethical-research-practice-ii-1/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

As a scientific researcher, you must present your work in an unbiased, original, and representative way. Without due care and attention, it is easy to drift over the boundary of what is acceptable and slip into habits that are unacceptable within the scientific community. This episode of Listen In with Karen O'Hanlon Cohrt defines plagiarism and presents some relevant statistics on this topic. She also explores self-plagiarism, demonstrates some good online plagiarism checkers, and showcases some examples of plagiarism and ways to avoid it. Despite the definition being straightforward, plagiarism is still a huge issue worldwide. It can be intentional and unintentional, which is why the key is to inform and educate researchers about what it entails. Watch the full presentation here: https://events.bitesizebio.com/ethical-research-practice-i-1/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Eukaryotic cell cultures respond to the slightest influence. Apart from the risk of contamination, minimal changes in cultivation parameters can affect their viability, growth, and cell metabolism. In this episode of Listen In, discover the measures you can take to improve the reproducibility of your experiments by incorporating Good Cell Culture Practice into your eukaryote culture routine. Explore the critical aspects of eukaryotic cell culture, including Mycoplasma test methods, effects of passage numbers, using Foetal Bovine Serum (FBS), and more! Watch the full presentation here: https://events.bitesizebio.com/everyday-culture-practice-improving-3/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Many scientists see the added value in using electronic pipettes and have already upgraded their pipette fleet. The benefits include: • Reduced strain on thumbs and arms by improving pipetting ergonomics. • Increased reproducibility of experiments with motor-operated pipetting cycles. • Easy and efficient handling of high sample throughput. In this episode of Listen In, learn why switching to electronic pipettes can be beneficial for a variety of applications in the lab. Get answers to your typical questions regarding their usage, reliability, and ergonomics, and discover new options with connected electronic devices and the Eppendorf Pipette Manager. Watch the full presentation here: https://events.bitesizebio.com/precision-and-efficiency-at-your/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Next-generation sequencing (NGS) has revolutionized the ‘omics’ era, with the rapid decoding of massive DNA sequences, supporting the investigation into various biological processes in human disease, genetic inheritance, immunity, cancer, and others. But generating high-quality NGS libraries is a labor-intensive process that requires experience, precision, and accuracy. Optimal, thorough sample preparation upstream of the sequencing process is essential to ensure the best possible results. In this episode of Listen In, get expert practical advice from Eppendorf on getting started with NGS in the lab, and discover how you can bypass pipetting-intensive protocols and automate your sample preparation with Eppendorf epMotion®—saving you time and effort and help you achieve reliable and reproducible sequencing results. Watch the full presentation here: https://events.bitesizebio.com/introduction-to-liquid-handling-for-1/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Centrifugation is the cornerstone of lab life, used in nearly every workflow, be it a routine task like pelleting cells or a dedicated assay for an important biomolecular target. That's why it's such a pain when they break down. But just because we use centrifuges every day doesn't mean there aren't important technical aspects to consider or no parameters you can tailor to your specific application. In this episode of Listen In, learn everything you need to know before next using a centrifuge. Let the experts from Eppendorf guide you through the difference between RPM and RCF, different rotor types, and dealing with aerosols. Plus, explore the best hardware for your intended experiment, get practical tips on prolonging their lifetime, and crucial safety information. Watch the full presentation here: https://events.bitesizebio.com/essentials-in-centrifugation-1/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Some scientific papers are unbelievable as they showcase experimental excellence that we did not think possible. These papers astound us with their groundbreaking findings and meticulous methodologies. However, alongside these unbelievably good papers, there are papers that are unbelievable due to the pervasive presence of statistical mistakes that undermine their credibility. Whether due to negligence, lack of expertise, or a rush to publish, these errors cast doubt on the validity of the reported results and conclusions. In this episode of Listen In, JJ Orban de Xivry delves into the ten most common statistical mistakes that plague scientific research (Makin and Orban de Xivry, eLife, 2019). With a focus on identifying these errors, he sheds light on the detrimental impact they have on the reliability of scientific findings. By identifying and rectifying statistical mistakes, researchers can ensure the foundation of their field of study. Watch the full presentation here: https://events.bitesizebio.com/ten-common-statistical-mistakes-to Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Your ultra-low temperature (ULT) freezer is not just a storage room for samples; it is your assurance for long-term scientific success and guards the results of your work. Therefore, every researcher should understand the importance of ULT freezers, know how to choose a reliable one, understand best practices for sample management, and have clear guidance on maintaining ULT freezers. In this episode of Listen In, learn the key considerations when buying a new ULT freezer for your lab and the best tips and tricks to prolong its lifetime. Plus, get advice on reducing the energy footprint of your ULT freezer and keeping your samples safe inside them. Watch the full presentation here: https://events.bitesizebio.com/how-to-keep-your-ult-freezer/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/